Association of Fc gamma receptor IIIB polymorphism with renal-allogrft in Chinese
2007/7/5
Gaosi Xua, b, Qiang Hea, Zhangfei Shoua, Huiping Wanga, Rending Wanga, Hua Jianga, Ying Chena and Jianghua Chena, 
, 
aKidney Disease Centre, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China
bShangrao Branch of Jiangxi Medical University, Shangrao 334000, China
Received 20 December 2006; revised 15 April 2007; accepted 30 April 2007. Available online 24 May 2007.
Abstract
Background
Several studies have identified FcγRIIIb (Fcgr3b) polymorphisms that determine susceptibility to autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. The objective of the study was to clarify whether FcγRIIIb allele polymorphism influence susceptibility to end stage 
renal
disease (ESRD) patients with renal transplantation.
Methods
DNA fragments were amplified by PCR-SSP using genomic DNA from 172 unrelated, healthy blood donors and 171 renal recipients in our Kidney Disease Centre. We performed direct sequencing using 20 PCR products of renal recipients carrying different FcγRIIIb forms according to the allele-specific PCR.
Results
FcγRIIIb NA1/NA2 genotype frequency showed significant difference between renal recipients and healthy controls (χ2 = 19.3, P < 0.005). In addition, the allele frequency also showed significant difference between the two groups. Lower frequency of genotype FcγRIIIb NA1/NA1 was observed in renal recipients than in healthy controls (χ2 = 5.06, P = 0.024). In comparison with nonrejectors, rejectors displayed no significant defference of FcγRIIIb NA1/NA2 genotype frequency, as well as allele frequency.
Conclusion
FcγRIIIb NA1/NA2 heterozygote genotype frequency was increased in ESRD patients in Chinese. The present findings showed that FcγRIIIb genotype related to the disease susceptibility, although FcγRIIIb polymorphisms did not affect the acute rejection.
Keywords: Fc gamma receptor; Polymorphism; Acute rejection; Renal allograft
Transplant Immunology
Volume 18, Issue 1, July 2007, Pages 28-31